Fig. 1
Zebrafish Zic2 is required during retinal morphogenesis. A: normal retinal morphology. B: retina exhibiting mild coloboma (*). C: retina exhibiting severe coloboma with periocular hemorrhaging (**). D: normal retinal morphology. E: bilateral coloboma in a severely affected embryo (**). F: mild, unilateral coloboma in an affected embryo. G: Penetrance and expressivity of coloboma is increased in progeny that lack maternal zic2b, derived from zic2agbt133/+; zic2bt104/zic2bt104 parents, compared to those from double heterozygous (zic2agbt133/+; zic2bt104/+) parents (see Table S1 for details). H, I: Both CRISPR- and TALEN-induced mutant alleles of zic2b are tightly associated with coloboma in MZ-zic2 embryos. Embryos in A-C are at 2–3 dpf, shown in lateral views, anterior to the left. Embryos in D-F are at 4 dpf, shown in anterior views, dorsal at the top.
Reprinted from Developmental Biology, 429(1), Sedykh, I., Yoon, B., Roberson, L., Moskvin, O., Dewey, C.N., Grinblat, Y., Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis, 92-104, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.