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Fig. 9
Remobilization of DsDELGT4 leads to imprecise excision and generates integrations with new reporter expression patterns. (A) Schematic representation of the remobilization experiment. Ac transposase mRNA was injected into one-cell stage embryos that show reporter expression. The injected embryos are raised to adulthood and mated with wild-type fish. Their progeny were examined for sequences flanking the original integration site and screened for new reporter expression patterns. (B–E) Remobilization of Ds in embryos of the single insertion line Tg(DsDELGT4)ws01961 (antisense strand, chr1:20205236-20205244) generates novel expression patterns (C–E) distinct from the original expression pattern (B). (F–H) Imprecise excision of DsDELGT4 by Ac-mediated remobilization causes mutations at the original integration site (location of the original insert is shown by black arrowhead in F), including deletions (F), indels (G), and insertions (H). Genomic DNA for sequence analysis (F–H) was obtained from the embryos of Tg(DsDELGT4)ws0310 (sense strand, chr21:17731630–17731722) and collected 24 hr after injection of Ac transposase mRNA.