ZFIN Image: Nguyen-Chi et al., 2017, Fig. S6

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Figure Caption

Fig. S6

Effect of tnfa morpholino on caudal fin regeneration (a) Fin images are representative transmitted light images of Ctrl morphants and tnfa morphants at 3dpA. (b) Corresponding regenerated fin length (mean ±s.e.m. from three independent experiments, ***P<0.001). (c) Schematic representation of the parabiosis experiment using tnfa MO or WT (CTRL) and Tg(mpeg1:GAL4/UAS:NTR-mCherry). Parabiotic larvae were treated with MTZ at 48 hpf and the caudal fin of Tg(mpeg1:GAL4/UAS:NTR-mCherry) was amputated at 3 dpf. (d) Representative fin images of mCherry fluorescence merged with transmitted channel at 6 hpA show NTRmCherry+ macrophages that are mainly depleted. White arrowheads show residual fluorescence in cells or cell fragments. (e) Fin images are representative transmitted light images at 3 dpA in tnfa morphants and CTRL. (f) Corresponding quantification of the regenerated fin length in indicated conditions (N_larvae=8-10 mean ± s.e.m., *P<0.05). In (a, d and e) dotted lines outline the fin, dashed arrows indicate the position of the initial transection. Scale bars = 100 μm.

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Acknowledgments

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