|
Fig. 7
Expression of fbrsl1 mRNA during zebrafish development. Whole-mount in situ hybridization analysis of fbrsl1-i2b transcript expression in wild-type embryos at different developmental stages: 10.3 hr (A and A'), 11 hr (B and B'), 12 hr (C–C''), 14 hr (D–D''), 18 hr (F, I, and I'), 24 hr (G, J, and J''), and 48 hr (H, K, and K'). (E and E') Double in situ hybridization with egr2a (krox20) as a second probe. (I, I'–K, and K'). Flat mount prep of embryonic brain with schematic presentation of brain subdivision at these developmental stages (I''–K''). Eyes were removed in (I'–K'). c, cerebellum; cg, cranial ganglia; de, diencephalon; dt, dorsal thalamus; e, epiphysis; fb, forebrain; hb, hindbrain; hy, hypothalamus; kv, Kupffer's vesicle; mb, midbrain; pt, posterior tuberculum; r, rhombomere; t, telencephalon; tc, tectum; tg, tegmentum; vt, ventral thalamus.