ZFIN Image: Pfefferli et al., 2017, Fig. S10

Image

Figure Caption

Fig. S10

Control experiments for careg lineage tracing analysis of heart and fin regeneration.

(a) Schematic representation of the transgenic strains and the experimental design for lineage tracing of careg-expressing primordial CMs during ventricle regeneration. (b-e) Immunostaining of uninjured ventricles treated with DMSO or 4-OHT using Tropomyosin (blue). Exposure to 4-OHT for 2 days is sufficient to label primordial CMs with zsYellow, as analysed at 7 days after the treatment (-1 day). (f) Schematic representation of the transgenic strains and the experimental design for lineage tracing of careg-expressing cells during fin regeneration. (g, h) Longitudinal fin sections at 0 and 4 dpa immunostaining for mCherry (red) and Zns5 (green). Exposure to 4-OHT for 1 day results in labelling of a few osteoblasts. (i-p) Live imaging of fins at different dpa after treatment with 4-OHT reveals only a few mCherry labelled cells in the stump and the regenerate. N ≥ 4.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.