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Fig. S11
Nrg2a can affect cardiomyocyte behavior via both autocrine and paracrine signals
(a-b) 2D ventral views (mid-sagittal sections) of 78 hpf Tg(myl7:EGFP-Hsa.HRAS) hearts from animals injected with tnnt2a MO (0.5 ng) and myl7:nrg2a-p2a-tdTomato DNA (5-10 pg). This low amount of DNA allows one to get single cardiomyocytes to overexpress the nrg2a transgene; arrows and arrowhead point to nrg2a-p2a-tdTomato expressing and nonexpressing cardiomyocytes, respectively; higher magnification of dashed box in a is shown in b; V: ventricle, At: atrium; scale bar, 50 μm.
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Full text @ Nat. Commun.