ZFIN Image: Wehner et al., 2017, Fig. S12

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Figure Caption

Fig. S12

CRISPR/Cas9-mediated disruption of col12a1a/b leads to impaired axonal regeneration.

(a) Transient CRISPR/Cas9-mediated gene editing of either col12a1a or col12a1b does not affect axonal regeneration (Fischer’s exact test: n.s. indicates not significant).

(b) Lack of axonal regeneration correlates with high gene editing efficiency of both col12a1 paralogues in CRISPR -manipulated larvae. Single larvae were analysed for axonal bridging at 2 dpl followed by restriction fragment length polymorphism (RFLP) analysis and determination of the gene edition efficiency index. Larvae without axonal bridge at 2 dpl show on average higher gene editing efficiency of col12a1a and col12a1b than larvae with axonal bridge (t-test: **P<0.01). Abbreviation: RE, restriction endonuclease. Error bars indicate s.e.m.

Acknowledgments

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