ZFIN Image: Paksa et al., 2016, Fig. S12

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Figure Caption

Fig. S12

PGCs are normally specified in embryos lacking the gut tube

One color (a and c) or two color (b and d) WISH on wild-type and sox32^ta56 embryos at 24hpf (a and c respectively) or 28hpf (b and d respectively). The gut is stained using foxa1 probe (a-d). In wild-type and sox32 mutant embryos the PGCs (insets) express both the early marker nos3 and the late marker piwil1. Arrowheads indicate the position of the gut (a-b), and no foxa1 expression is detected in the mutants (c-d). Number of embryos examined in a: N=40, b: N=20, c: N=9 and d: N=5. (e-f) PGCs are labeled with vasa-gfp mRNA, showing the characteristic granule formation in PGCs (insets in e and f respectively). e: N=14 and f: N=15. Scale bar 50μm. Dorsal views are shown. Anterior is up.

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