ZFIN Image: Paksa et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Single guide RNAs efficiency in generating mutations

(a) Functionality of a set of control sgRNAs, demonstrated by loss of pigmentation in majority of the treated embryos at 3dpf as compared with uninjected embryos. Scale bars 200μm. (b) Efficiency of sgRNAs designed for lpp genes. Generation of the mutations by one sgRNA per gene-specific set (right panels), as evaluated by sequencing, compared with the results obtained using a control sgRNA (left panels) targeting the tyrosinase gene (G416 in Supplementary Table 3). Ambiguous sequence at the lpp sgRNA-binding region in the sequencing results confirms the efficiency of the genome-editing procedure.

Acknowledgments

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