ZFIN Image: Ke et al., 2017, Fig. 5

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Fig. 5

Gβ1 signaling is critical for neutrophil motility. Epifluorescence time-lapse experiments were performed on neutrophils in the head region (green box) of 72 hpf-Tg(mpx:GFP) embryos, using ×5 (A-D) and ×20 (E-G) objectives. (A) Representative image showing GFP-expressing neutrophils in the head region. (B-C) 15-min tracks of neutrophil migration, from time-lapse movies of control (B) and gnb1ab MO1-injected (C) Tg(mpx:GFP) embryos (Supplementary Movie 4). (D) Speed of neutrophil migration in the indicated embryos (69 neutrophils in 6 control embryos, 60 neutrophils in 6 gnb1ab MO1-injected embryos). (E-F) Snapshots from 2-min movies of control (E) and gnb1a/b MO1-injected (F) Tg(mpx:GFP) embryos, showing the morphological changes in neutrophils (Supplementary Movie 5). (G) Changes in length-width ratio (LWR) of neutrophils over time (17 neutrophils in 2 control embryos and 11 neutrophils in 4 gnb1ab MO1-injected embryos were analyzed, *P < 0.0001).

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Acknowledgments

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Reprinted from Developmental Biology, 428(1), Ke, W., Ye, D., Mersch, K., Xu, H., Chen, S., Lin, F., Gβ1 is required for neutrophil migration in zebrafish, 135-147, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.