ZFIN Image: Förster et al., 2017, Fig. 1

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Figure Caption

Fig. 1

A candidate-based BAC screen identifies new genetic tools for targeting neuronal subpopulations. (a) Workflow to establish BAC transgenic fish lines. WT, wild-type. (b) Plasmid constructs used for BAC recombineering. KanR, kanamycine-resistant gene; pA, polyadenylation signal. (c) Relationship between the genomic insert length of all BAC constructs (7 Gal4VP16 BACs, 39 Gal4VP16-Bleeding Heart BACs, and 12 Cre-Cold Heart BACs) and their founder rate (percentage of germline founders out of total adult fish screened). Note that the founder rate is not correlated with the genomic insert length (R-squared = 0.0007). (d) Dorsal view of 6 dpf old larval brains showing the live expression pattern of ten selected transgenic Gal4 lines (green; Dendra-kras, GCaMP6s or EGFP). Brains have been registered via co-expression of HuC:lynTagRFP-T (magenta). Scale bar, 100 µm.

Acknowledgments

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