ZFIN Image: Lin et al., 2017, Fig. 6

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Figure Caption

Fig. 6

Change of expression levels of miR-206, Rtn4al, Cxcr4a or Thbs3a fails to epithelialize somites in zebrafish embryos. (a) A diagram depicts that centrosomes of epithelial cells at the somite boundary are localized apically when epithelial cells undergo MET. (b) WT embryos at 20 hpf; (c) knockdown of miR-206, (d) overexpression of rtn4al, (e) knockdown of cxcr4a and (f) overexpression of thbs3a. Fibronectin labelled with green fluorescent signal was used to mark the somite boundary; Phalloidin labelled with red fluorescent signal was used to mark F-actin, while γ-tubulin labelled with blue fluorescent signal was used to mark centrosomes. (b–f) Three fluorescent signals were merged; (b′–f′) were amplified from the corresponding panels (b–f). White arrowheads indicate centrosomes not localized apically in the epithelial cells of the defective somite boundary.

Figure Data

Phenotype details

Acknowledgments

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