ZFIN Image: Lim et al., 2017, Fig. S2

Image

Figure Caption

Fig. S2

Live chordamesoderm transition and vacuole formation characterization of cavin1b-/- mutant embryos, related to Figure 1:

(A) Live imaging of chordamesoderm (c) and adjacent somites (s) in 12-somite stage WT and cavin1b-/- embryos labeled with BODIPY FL C5-Ceramide. Bar, 100 μm.

(B) Representative live dorsal images of 2 dpf (top row) and 4 dpf (bottom row) notochord vacuoles of WT and cavin1b-/- embryos labelled with BODIPY TR methyl ester. Bar, 100 μm.

(C-F) Quantitation of notochord vacuole area and number per 100 μm length of the notochord for 2 dpf (CD) and 4 dpf (E-F) WT and cavin1b-/- embryos. For cavin1b-/- notochords, images containing lesions were excluded from this quantitation. Number of fish used for 2 dpf: WT=19 and cavin1b-/-=23. Number of fish used for 4 dpf: WT=19 and cavin1b-/-=17. 4 clutches per group. Data are presented as mean±SD. ns=P> 0.05. P values were determined using two-tailed, unpaired t-tests.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.