ZFIN Image: Murphy et al., 2017, Fig. 5

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Fig. 5

Brd2a knockdown has opposite effects on cell death at start and end of segmentation. Embryos were analyzed for apoptotic nuclei using TUNEL followed by laser-scanning confocal microscopy and quantified using SimplePCI computer analysis software. A total of 24 embryos and three clutches per treatment (uninjected WT, 8 ng brd2aMO1-injected, and 8 ng control MOc-injected) were assessed. Light micrographs with accompanying dark field maximum projection composite images show representative wildtype (A,B), brd2aMO1-injected (C,D), and control MOc-injected (E,F) embryos at bud stage; and wildtype (G,H), brd2aMO1-injected (I,J), and control MOc-injected (K,L) embryos at prim 5 stage. Cell death appears relatively reduced in morphants at bud stage (D vs. B and F), but increases dramatically compared to controls at prim 5 (J vs. H and L).

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Acknowledgments

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Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.