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Fig. S10
Splicing padi2 morphants display vascular defects at 48 hpf.
A) RT-PCR of total RNA extracted from padi2 morphants show the newly designed MO causes an aberrant splicing resulting to partial retention of intron 6 within the transcript. B) Significant vascular defects in a new padi2 MO compared to standard-injected MO (negative) control siblings (representative figures from 12 injections, repeated 3 times). Note the gaps present in the intersegmental vessels suggesting missing or abnormal formation.
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