ZFIN Image: Khajavi et al., 2017, Fig. 8

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Figure Caption

Fig. 8

Human PADI2 mRNA can rescue vessel defects in zebrafish padi2 morphants.

A) Significant vascular defects (arrows) are present in padi2 MO (1.5 ng/embryo) (b) compared to standard (sham-injected) MO control (1.5 ng/embryo) (a) and human PADI2 (hPADI2) mRNA only (225 pg/embryo) (c). Co-injection of hPADI2 mRNA (225 pg/embryo) rescues padi2 MO (1.5 ng) vascular defect. (d) Magnification: x10. B) Bar graph of normal and defective ISVs in four different groups, showing average ±SD from four independent experiments. Missing and abnormal formation of ISVs are considered as defective vessels. The number of defective vessels is significantly increased with the injection of padi2 MO (* and ** denotes P<0.001 and P<0.005 respectively.) C) Citrullination levels in zebrafish. The level of deiminated protein is partially rescued in padi2 morphants with the co-injection of hPADI2 mRNA.

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Acknowledgments

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