Fig. 8
Human PADI2 mRNA can rescue vessel defects in zebrafish padi2 morphants.
A) Significant vascular defects (arrows) are present in padi2 MO (1.5 ng/embryo) (b) compared to standard (sham-injected) MO control (1.5 ng/embryo) (a) and human PADI2 (hPADI2) mRNA only (225 pg/embryo) (c). Co-injection of hPADI2 mRNA (225 pg/embryo) rescues padi2 MO (1.5 ng) vascular defect. (d) Magnification: x10. B) Bar graph of normal and defective ISVs in four different groups, showing average ąSD from four independent experiments. Missing and abnormal formation of ISVs are considered as defective vessels. The number of defective vessels is significantly increased with the injection of padi2 MO (* and ** denotes P<0.001 and P<0.005 respectively.) C) Citrullination levels in zebrafish. The level of deiminated protein is partially rescued in padi2 morphants with the co-injection of hPADI2 mRNA.