ZFIN Image: Khajavi et al., 2017, Fig. S11

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Figure Caption

Fig. S11

Dosage Titrations for padi2 MO and hPADI2 mRNA injection.

A) Poorly conserved region at the 5' ATG start site between zebrafish padi2 and human PADI2 mRNA sequence. The designed MO targets the ATG start site (highlighted) of zebrafish padi2. MOs differ by more than five out of 25 nucleotides from their target sequence do not interfere with translation of the targeted mRNA. B) By titrating the dose of human PADI2 (hPADI2) mRNA, we determined that injection of up to 225 pg into early embryos did not cause any phenotypic changes or lethality. Notably, injection of high copy number of hPADI2 mRNA (>250 pg) alone results in significant toxicity in zebrafish.

Acknowledgments

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