Fig. 4

Fig. 4

Loss of Ta and Tbx16 Leads to Downregulation of Endodermal Specifiers and Reduction of Endodermal Progenitors during Gastrulation

(A) qPCR analysis of mixl1, gata5, and sox32 at 50% epiboly (5.3 hpf) in single and double ta/tbx16 morphants. All genes are significantly downregulated in double morphants; ^∗p ≤ 5 × 10⁻²; Student’s t test. Data are represented as mean ± SEM. See also Figure S2.

(B) WISH analysis of sox32 in single and double morphants at germ ring stage (5.7 hpf). Arrowhead, YSL expression; ^∗, loss of endoderm expression.

(C) Microarray analysis at 90% epiboly (9 hpf) indicates downregulation of cxcl12a/b. Data are represented as mean ± SEM.

(D) Immunological and WISH analysis of a sox17:eGFP transgene and endogenous sox17 expression at 90% epiboly (9 hpf) in single and double morphants.

(E) Cell numbers identified by immunostaining and WISH in (D). Cell numbers are representative of at least 20 embryos per condition. ^∗p ≤ 1 × 10⁻⁸; ^∗∗p ≤ 1 × 10⁻²⁰; Student’s t test. Data are represented as mean ± SEM.

See also Figure S3.