ZFIN Image: Holland et al., 2016, Fig. 2

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Fig. 2

HS1BP3 regulates autophagy in zebrafish.

(a) Representative confocal images of GFP-LC3 puncta (autophagosomes) in the trunk area of GFP-LC3 transgenic zebrafish embryos injected with control morpholino (C), Hs1bp3 translational-blocking morpholino (K), and the human Hs1bp3 mRNA coinjected with the morpholino (R) and imaged at 2 dpf with or without pre-treatment with chloroquine (10 μM) for 6 h. Scale bars, 10 μm. (b) GFP-LC3 puncta were counted in the trunk region (marked in d) of the transgenic zebrafish embryos at 2 dpf (mean±s.e.m., n=3). Total of 7–13 embryos were used for each condition per experiment. *P<0.05, **P<0.01, ***P<0.001, by Student’s t-test. (c) Representative immunoblotting of Hs1bp3 and Tubulin in whole lysates of zebrafish embryos at 2 dpf, treated with or without chloroquine for 6 h before harvest. (d) Representative light fluorescent microscopy images of whole embryos at 2 dpf. Scale bars, 300 μm.

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Acknowledgments

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