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Fig. 2
Morpholino-mediated pitpnaa knockdown in zebrafish elicits dose-dependent changes in morphology and signaling. (A) Phase contrast images of sapje larvae injected with pitpnaa MO or control MO at 1 and 4 dpf. Embryos injected with 3 ng of pitpnaa MO at the one-cell stage showed abnormal development and were not viable, whereas 1 ng of pitpnaa MO did not negatively impact development. (B) Western blot showing decreased Pitpna expression with increasing doses of pitpnaa MO injected into sapje embryos. Protein lysates were harvested from sapje larvae at 4 dpf. Experiment performed in triplicate, n = 300 per replicate.