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Figure Caption
Fig. 2 S1
Putative NECs in the skin of embryonic zebrafish are not neural crest-derived.
(a–b’) Three-dimensional rendering of the eye of a 3-dpf Tg(crestin:creERT2);Tg(-3.5ubi:loxP-GFP-loxP-mCherry) embryo, immunostained in whole-mount for serotonin and mCherry. Serotonergic cells are scattered in the epidermis over the eye, as reported (Coccimiglio and Jonz, 2012), but none is mCherry-positive, i.e., neural crest-derived (arrowheads). For associated z-stack movies, see Videos 1 and 2. Scale-bars: 100 μm in a; 50 μm in b.
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