Fig. 6

Fig. 6

cos2/kif7^mw406 exhibit delayed retinal neurogenesis. A. At 48hpf, embryos were incubated with EdU for 15-min to label cells in S-phase of the cell cycle. Nearly every presumptive photoreceptor in the ONL is labeled with the EdU (green). All nuclei were labeled with TO-PRO (blue). B. 50hpf embryos were given an identical EdU treatment. cos2/kif7^mw406 presumptive photoreceptors remained strongly labeled by the EdU, while control and osm-3/kif17^mw405 presumptive photoreceptors do not. C. EdU treatment of 55hpf embryos shows that although decreased from previous time-points, cos2/kif7^mw406 presumptive photoreceptors remained labeled by the EdU. Scale bar is 25 µm. D. (Top) Quantification of the number of presumptive photoreceptor nuclei labeled by the EdU treatment as a measure of the total length of ONL. The number of EdU positive nuclei were counted among several images along a total average ONL length of 408±26 µm for five biological replicates at each timepoint for each genotype. At 48hpf, presumptive photoreceptors of each genotype are strongly labeled by EdU, while cos2/kif7^mw406 labeling remains significantly increased through 55hpf. (Bottom) Quantification of the number of inner retinal cells (including INL and GCL) labeled by the EdU treatment as a measure of the total inner retinal area. The number of EdU Positive nuclei were counted among several images within a total average inner retina area of 14000±500 µm² for five biological replicates at each timepoint for each genotype. Inner retinal cells have increased EdU incorporation between 48hpf and 55hpf.