ZFIN Image: Lewis et al., 2017, Fig. 4

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Figure Caption

Fig. 4

cos2/kif7^mw406 results in a OS developmental delay. A. Plastic histology at 60hpf reveals OS are virtually absent in cos2/kif7^mw406 retinas compared to the presence of numerous OS in controls (arrows). B. At 72hpf, cos2/kif7^mw406 have few and small OS compared to controls. C. At 96hpf, cos2/kif7^mw406 retinas have comparable OS densities, although the size still appears smaller. Scale bar is 10 μm. D. (Top) Quantification of the average area of an individual OS during the first 4dpf. At all time-points measured, the decrease in size of cos2/kif7^mw406 OS is statistically significant. For both genotypes, 100 total OS were measured for each of five biological replicates at each timepoint. (Bottom) Quantification of the average number of OS per length of RPE. During the initial 72hpf, the decrease in numbers of both cos2/kif7 mutants is statistically significant. For all genotypes, a total length of 250 µm of RPE was measured for each of five biological replicates at each timepoint.

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Acknowledgments

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Reprinted from Developmental Biology, 425(2), Lewis, T.R., Kundinger, S.R., Pavlovich, A.L., Bostrom, J.R., Link, B.A., Besharse, J.C., Cos2/Kif7 and OSM-3/Kif17 Regulate Onset of Outer Segment Development in Zebrafish Photoreceptors Through Distinct Mechanisms, 176-190, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.