ZFIN Image: Lee et al., 2017, Fig. S8

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Figure Caption

Fig. S8

Generation of cnbp morphants. (A) Schematic of antisense morpholino oligonucleotides for cnbp in Zebrafish. (B) RT-PCR analysis of cnbp in control or cnbp morpholino-treated zebrafish embryos at 24 hpf to assess depletion efficiency. (C) Images highlighting the morphological defects of cnbp morphants during development. Scale bars, 500 μm. (D) Infiltration of neutrophils in control and cnbp morphants after LPS treatment. In cnbp-depleted zebrafish, neutrophils fail to migrate to the inflammatory foci in the yolk of LPS-injected larvae. LPS- or PBS-injected zebrafish larvae were collected at 5 h post-injection. For Sudan Black (SB) staining, the fixed larvae were permeabilized with ethanol, after which the yolk regions were stained with 0.03% SB. Migration of SB-stained neutrophils was observed under a dissecting microscope. Images were captured and analyzed using a mono-camera and NIS-Elements software, respectively. Scale bars, 500 µm, *P<0.01 (Student’s t-test). Data are representative of three independent experiments.

Acknowledgments

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