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Fig. 5
Genoarchitectonic signatures of the hypothalamo-prethalamic primordium (HyPTh) (I).
(A-A′′) Combined Fezf-Gbx expression defines a Fezf-positive rostral archencephalic prototagma (ARCH) territory identified as HyPTh (as per Fig 3K and 3L, for reference). (B-B′′) Rx is specifically expressed throughout the entire HyPTh primordium. (C-C′′) Neural expression of FoxD was detected only in the basal and floor plates of HyPTh. (D-E′′) Nkx2.2 is expressed in the Rostral-HyPTh and Interm-HyPTh domains but not in Caudal-HyPTh (D-D′′), as observed by a large gap in a double in situ hybridization for Nkx2.2 and Gbx (E-E′′). (F-G′′) Similarly, Meis mRNA is only detected in the basal plate of the Rostral and Intermediate domains of HyPTh (F-F′′), leaving a gap of expression when combined with IrxC (G-G′′). Further expression of Meis is also detected in specific deuteroencephalic prototagma (DEU) areas (F-F′′). (H-H′′) Lef is expressed in the basal plate of Rostral-HyPTh and Interm-HyPTh. Expression patterns correspond to lateral (A-H) or dorsal views (A′-H′) at the 21 h post fertilization (hpf) embryonic stage and are represented in schematics dorsal views (A′′-H′′). Somites (dotted lines) were used as main landmarks to localize the position of the patterns analyzed in the late neural plate. Scale bar: 50 μm.