Fig. 1 S1

Fig. 1 S1

Expression of BRAF^V600E does not affect proliferation of larval thyrocytes.

(A–B) Representative confocal images stacks of 5 dpf fixed transgenic tg-TOM and tg-BRAF^V600E-TOM larvae stained with Sytox Green Nucleic Acid Stain; arrows indicate the nuclei of TdTomato-positive cells. (C) Quantification of the total number of thyrocytes (n = 10 for both genotypes). (D–E) Representative confocal images of tg-TOM and tg-BRAF^V600E-TOM larvae incubated with BrdU from 3dpf to 5dpf. Immunofluorescence performed with an anti-BrdU antibody, arrows indicate TdTomato-positive BrdU-positive nuclei. (F) Quantification of the TdTomato-positive BrdU-positive thyrocytes (n = 4 and n = 3 for tg-TOM and tg-BRAF^V600E-TOM, respectively) (G) Representative images of 5 dpf tg-TOM and tg-BRAF^V600E-TOM thyroid obtained using Imaris software. (H) Quantification of the thyroid volume (18040 ± 15199 μm³ and 14841 ± 6422 μm³ for tg-TOM and tg-BRAF^V600E-TOM). Ns: not significant, two-tailed Student's t-test.