ZFIN Image: Keightley et al., 2017, Fig. S3

Image

Figure Caption

Fig. S3

Genetic proofs validate identification of Zbtb11 from positional cloning (supports Fig. 1d)

a. Phenocopy of mne hydrocephalus phenotype (open arrow) and microphthalmia (solid arrow) phenotypes and neutrophil-depletion phenotypes (fluorescence images) by antisense morpholino oligonucleotide knockdown of Zbtb11 in WT embryos;

b. Enumeration of neutrophils in morphant embryos represented in a; n numbers are shown within columns;

c. Rescue of mne hydrocephalus (open arrow) and microphthalmia (solid arrow) phenotypes and neutrophil-depletion phenotype (grey arrows in fluorographs) by overexpression of WT but not C116S Zbtb11 mRNA in mne embryos;

d. Enumeration of neutrophils in morphant embryos represented in c; n numbers are shown within columns;

e. Additional zbtb11 allele generated by CRISPR/Cas9 locus genome editing. Shown is sequence for WT zbtb11 and a zbtb11^Cr CRISPR allele with predicted premature stop codon at amino acid 56;

f. Non-complementation of mne allele by zbtb11^Cr allele in mne;zbtb11^Cr transheterozygotes (black arrow, microphthalmia; grey arrows, neutrophils); Panels a, c, f scale bar = 200 μm.

Acknowledgments

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