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Fig. S8
BMS-833923 potently inhibits Hedgehog/Smoothened signaling in zebrafish. (AR) Whole mount images of Kaede fluorescence dynamics 18 hours post-conversion in ptch2:Kaede animals treated with either DMSO, cyclopamine (20 mg/kg), BMS-833923 (50 mg/kg), Vismodegib (75 mg/kg), Erismodegib (50 mg/kg), SANT-1 (50 mg/kg), Taladegib (8.25 mg/kg), Glasdegib (50 mg/kg), or PF-05274857 (100 mg/kg). Small molecules were administered at 72 hpa, Kaede protein was photoconverted on half of the fin using 405 nm light at 78 hpa, and imaging was performed at 96 hpa. Newly expressed Kaede protein is green, photoconverted Kaede is magenta. At least four fish were analyzed from each group. The white brackets indicate the domain of new Kaede expression in the 18 hours post-photoconversion and the magenta arrows denote previously Kaede-positive epidermis. Dashed yellow lines show the position where fins were amputated. Scale bars: 500 μm.