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Fig. 4
BMS-833923, a newly identified potent zebrafish Smoothened inhibitor, shows that Hedgehog/Smo signaling does not impact fin regenerative outgrowth. (A-L′) Whole-mount fluorescence images of 96 hpa fins from ptch2:Kaede fish 18 h after Kaede photoconversion and 5 h after EdU injection. Regenerating fish were treated for 24 h with DMSO (A-D′), cyclopamine (E-H′) or BMS-833923 (I-L′). Newly produced Kaede and converted Kaede are green and magenta, respectively, in overlay images (C,G,K). Magenta arrows indicate cells with converted Kaede. White brackets mark the domain of new Kaede expression in the 18 h since photoconversion. (D,H,L) Fins from the same fish shown for Kaede fluorescence processed to reveal EdU-incorporating nuclei in green. (D′,H′,L′) Confocal stack images showing EdU incorporation at the distal aspect of single re-forming rays. The timeline at the top of the figure details the experimental design. Scale bars: 500 μm.