ZFIN Image: Seberg et al., 2017, Fig. 5

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Figure Caption

Fig. 5

tfap2a and mitfa display a genetic interaction in zebrafish.

(A-I) Compared to wildtype embryos at 72 hpf (A), melanocytes in mitfa^z25/z25 mutant embryos (B) are dark but punctate, and melanocytes in mitfa^w2/z25 trans-heterozygous embryos (C) are pale, punctate, and reduced in number. While heterozygous mutation of tfap2a in the wildtype background (tfap2a^+/-) (D) does not result in a melanocyte phenotype, mitfa^z25/z25;tfap2a^+/- mutants (E) and mitfa^w2/z25;tfap2a^+/- mutants (F) display a loss of melanocytes in the ventral stripe (brackets). Similarly, the well-characterized phenotype of fewer, paler melanocytes in tfap2a^-/- null mutants (G) is more pronounced in both the mitfa^z25/z25 mutant background (H) and the mitfa^w2/z25 mutant background (I). (J, K) Counts of ventral stripe melanocytes (see brackets in A-I) showed no difference between wildtype and tfap2a^+/- embryos (J, n = 10+), while tfap2a^+/- embryos in both mitfa mutant backgrounds had significantly fewer melanocytes in the ventral stripe (K, n = 4–8). One-way ANOVA: *p<0.05, **p<0.01, ****p<0.0001.

Figure Data

Phenotype details

Acknowledgments

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