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Fig. 7
Quantification of Notch reporter differences in m618 and baz1 mutants at 48 hpf.
A-F) confocal acquisitions of WT control (A,D) and mutant (B, m618; E, baz1) trunk regions, followed by Notch reporter (NRE:EGFP) fluorescence analysis (C,F). A slight increase of reporter signal is detected in the neural tube (nt) and dorsal root ganglia (drg, arrowhead) of m618 mutants (B) compared to controls (A), while a decrease of signal is detected in the same regions of baz1 mutants (compare E with D). Relative fluorescence intensity (RFI) in aorta (a) and intersomitic vessels (v) appears unmodified in all conditions. n = 6 measurements per condition. n.s. = not significant; ** = p<0.01; *** = p<0.001.