ZFIN Image: Delfino-Machín et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Medial pathway neural precursors undergo precocious and supernumerary differentiation into neurons in baz1 mutants.

Confocal images of developing trunk DRGs of WT (A, D, G, J), baz1 (B, E, H, K) and m618 mutants (C, F, I, L) showing Elav1/Hu (red) and sox10:GFP (green) at each of 36 (A-C), 42 (D-F), 48 hpf (G-I) and 5 dpf (J-L). Arrowheads indicate subset of Elav1/Hu⁺ DRG sensory neurons. M-P) Counts (mean±s.d.) of trunk (Tr) and tail (Ta) and total (TOT) Elav1⁺ cells in DRGs of baz1 (yellow) and m618 (blue) mutants and their respective WT siblings. Significantly elevated numbers of neurons are indicated (two-tailed Student’s t test; **, p<0.01; ***, p<0.001). Note in panels J-L) that variable prominence of Elav1/Hu detection in spinal cord is an artefact of antibody penetration into CNS at this late stage. Scale bar, 50 μm.

Acknowledgments

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