ZFIN Image: Zhang et al., 2017, Fig. 6

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Figure Caption

Fig. 6

A myomaker promoter fragment with multiple E-boxes is sufficient to direct reporter expression in the fast-twitch muscle lineage. A: Schematic diagram of 5′ genomic region of myomaker. The black and white boxes represent Exon 1 and 5′ UTR respectively, while the red lines indicate locations of the 23 E-box sequences. B: The 3.278 kb promoter fragment is sufficient to drive GFP expression (detected with anti-GFP staining) in fast-twitch muscle cells (labeled with F310 antibody), as observed in both 20-somites (Top panel) and 2 d.p.f. embryos (Bottom panel). Nuclei and cell membranes are identified by DAPI and anti-β-catenin antibodies. Scale bar=20 µm.

Acknowledgments

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Reprinted from Developmental Biology, 423(1), Zhang, W., Roy, S., Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo, 24-33, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.