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Fig. 1
Zebrafish stat3 mutants generated using TALEN method are strong/null alleles.
(A) Design of TALEN pair targeting zebrafish stat3 gene (yellow filled text). (B) Sequence alignments and schematics of Stat3 proteins encoded by stl27, stl28 and sa15744 alleles. (C) Expression patterns of stat3 transcripts detected by WISH in WT and MZstat3 embryos (lateral views). Arrowhead, nm, neuromast. (D) RT-PCR analysis of stat3 RNA levels in WT embryos at 1.3, 6, 8.3 and 24 hpf. gapdh was used as an internal control. (E) qRT-PCR analysis of stat3 transcript levels in WT and MZstat3 embryos normalized to gapdh. (F) Western blot detecting total Stat3 in WT, MZstat3 and MZstat3 embryos overexpressing Flag-tagged Stat3 at 6 hpf. β-actin was used as a loading control. See also S1 Fig.