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Fig. 9
The ALPM engages in endoderm-dependent subduction. Snapshots from epifluorescence time-lapse movies of control and gna13a/b MO-injected Tg(sox17:memCherry)/(hand2:EGFP) embryos at 10-20 s (supplementary material Movie 6). Dorso-anterior views. (A-L) Overlays of anterior memCherry-labeled endoderm (red) and Hand2:GFP-labeled ALPM (green). (A′-L′) Hand2:GFP-labeled ALPM. (A″-L″) memCherry-labeled endoderm. Yellow arrowheads indicate myocardial cells (MCs); red arrowheads indicate dorsalmost region of LPM cells; yellow and red lines indicate gap between the two myocardial populations and the dorsalmost region of LPM cells. Scale bars: 100 µm.