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Fig. 4
Otp paralogs have differential effect on hypothalamic oxt expression.
(A?D) Representative high-resolution micrographs of 5 day-old embryos that were subjected to whole mount in situ hybridization with oxt mRNA probe (dorsal view, anterior to the top). oxt-positive neurons in the posterior tuberculum (PT) of otpa?/? mutants are marked by a dotted rectangle (B). Scale bar, 20 µm. (E,F) Box and whisker plots showing OXT cell number counts in the neurosecretory preoptic area (NPO; E) and in the PT (F) in various genotypes. The numbers of embryos used for the quantification are indicated above each box. In the NPO, otpa+/+;otpb+/+ fish differed significantly only from otpa?/?;otpb+/? (*p=0.013) and otpa?/?;otpb?/? (***p=0.000). In the PT otpa+/+;otpb+/+ differed significantly (***p=0.000) only from otpa?/?;otpb+/? and otpa?/?;otpb+/+.