ZFIN Image: Webster et al., 2017, Fig. 4

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Figure Caption

Fig. 4

Adult homozygous dmrt1 mutants have disorganized testes with reduced germ cells. A-F) H&E staining of adult male gonads. A) Wild-type adult testes are organized into tubules, with cysts of differentiating germ cells surrounding lumens containing spermatozoa (sz). B and C) Dmrt1^S10X mutants form enlarged testis tubules with reduced numbers of germ cells and no spermatozoa. Tubules have large lumens devoid of germ cells (e.g. black asterisk). D) dmrt1^E3ins and E) dmrt1^C41X mutant testes exhibit similar phenotypes, often displaying increased stroma (e.g. arrows in D, F), with few germ cells and retention of ovarian cavities (white arrowheads). Neither genotype organizes testis tubules nor forms lumens. F) A transheterozygous dmrt1^E3ins/C41X mutant looks highly similar to both dmrt1^E3insand dmrt1^C41Xhomozygotes. Spermatogonia=black arrowhead; lumen filled with spermatozoa=sz; lumen lacking spermatozoa=black asterisk; stroma=arrow; ovarian cavities=white arrowhead. Scale bar=50 µm.

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 422(1), Webster, K.A., Schach, U., Ordaz, A., Steinfeld, J.S., Draper, B.W., Siegfried, K.R., Dmrt1 is necessary for male sexual development in zebrafish, 33-46, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.