ZFIN Image: Heanue et al., 2016, Fig. S1

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Figure Caption

Fig. S1

ret^hu2846/+ larvae exhibit phenotypic variation.

7dpf larvae immunostained with the HuC/D antibody to visualize ENS neurons in WT (A) and ret^hu2846/+ (B-D) larvae. Asterisks indicate end of the gut tube (anal pore), and arrows denote the position of the most distal HuC/D⁺ neuron. At 7dpf, WT (ret^+/+) larvae exhibit HuC/D⁺ ENS neurons along the full length of the gut (A). Some ret^hu2846/+ larvae also show full colonization of the gut by HuC/D⁺ ENS neurons (B), and are referred to as ‘non-phenotypic’ or ret^hu2846/+ NP. Other ret^hu2846/+ larvae show varying extents of distal regions lacking HuC/D⁺ ENS neurons (C, D), and are referred to as ‘phenotypic’ or ret^hu2846/+ P. High-resolution images of full gut length was constructed by tiling two overlapping micrographs.

Acknowledgments

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