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Fig. S7
Isolation of ECs from zebrafish embryos and measurement of candidate gene expression by RT-PCR. (A) ECs were isolated from 26 hpf flk1:EGFP-NLS zebrafish embryos using fluorescence-‐activated cell sorting (FACS). (B) Expression of EC marker cdh5, house-‐keeping gene beta-‐actin (bact2) and candidate genes of interest was assessed in ECs (GFP+) by RT-‐PCR followed by analysis of product by gel electrophoresis, while GFP-‐ cells were used as a control. The DNA fragment sizes are indicated in base pairs next to the DNA ladder (L).
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Full text @ Arterio., Thromb., and Vas. Bio.