ZFIN Image: Noack Watt et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Pharyngeal arch size is reduced in polr1c^-/- and polr1d^-/- mutants.

(A-C) Immunostaining of 36hpf fli1a:egfp labeled control, polr1c^-/- and polr1d^-/- mutant embryos with Zn-8 (red), which labels the endodermal pouches, revealed comparatively normal pharyngeal arch and pharyngeal pouch patterning. Pharyngeal arches 1–5 are indicated. (D-F) fli1a:egfp labeling of post-migratory NCC illustrates an overall reduction in pharyngeal arch size in polr1c^-/- and polr1d^-/- mutants. Pharyngeal arches 1 and 2 are outlined in red. (G-H) Quantification of pharyngeal arches 1 & 2 (red arrows, D-F) volume revealed a reduction in polr1c^-/- (G) and polr1d^-/- (H) mutants. Scale bar = 100 μm. * = p < 0.05 and error bars represent 95% confidence intervals.

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Phenotype details

Acknowledgments

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