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Fig. S1
Fzd3a has a cell-autonomous function in FBMN migration.
(A-B) Live confocal images of 48 hpf chimeric embryos with anterior to the top. Transplant conditions are indicated as donor?host. Pk1bMO host embryos were used because they have normal neuroepithelial planar polarity but unmigrated FBMNs; this prevents donor-derived FBMNs from being carried to r6 by migrating host neurons in a PCP-independent manner. Cascade blue-dextran marks all donor-derived cells (blue), Tg(isl1:mRFP) marks host FBMNs (magenta) and Tg(isl1:GFP) marks donor-derived FBMNs (green). Histograms on the right indicate the percent of donor-derived FBMNs at 48 hpf that failed to migrate (rhombomere (r)4), partially migrated (r5) or fully migrated (r6) and numbers indicate the number of FBMNs represented in each bar. N indicates the number of chimeric embryos and n indicates the number of FBMNs scored in each condition. Brackets indicate rhombomere positon. Scale bar: 50 ?m.