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Fig. S1
Fzd3a has a cell-autonomous function in FBMN migration.
(A-B) Live confocal images of 48 hpf chimeric embryos with anterior to the top. Transplant conditions are indicated as donor→host. Pk1bMO host embryos were used because they have normal neuroepithelial planar polarity but unmigrated FBMNs; this prevents donor-derived FBMNs from being carried to r6 by migrating host neurons in a PCP-independent manner. Cascade blue-dextran marks all donor-derived cells (blue), Tg(isl1:mRFP) marks host FBMNs (magenta) and Tg(isl1:GFP) marks donor-derived FBMNs (green). Histograms on the right indicate the percent of donor-derived FBMNs at 48 hpf that failed to migrate (rhombomere (r)4), partially migrated (r5) or fully migrated (r6) and numbers indicate the number of FBMNs represented in each bar. N indicates the number of chimeric embryos and n indicates the number of FBMNs scored in each condition. Brackets indicate rhombomere positon. Scale bar: 50 μm.