ZFIN Image: Bielczyk-Maczyńska et al., 2015, Fig. S1

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Figure Caption

Fig. S1

nol9 mutation affects the development of liver and intestine but not of jaw cartilage.

(A) Representative pictures of 96 hpf larvae stained by WISH against the liver marker prox1 (black arrow). Magnified images of the liver are shown. (B) Quantification of the prox1 in situ hybridization data. The average prox1-positive area is decreased in nol9^{sa1022/sa1022} mutants (n = 13) compared to wild-type siblings (n = 25). Data are represented as the average +/- SEM. Two-tailed Student’s t test, ***, p<0.001. (C) Representative images of 96 hpf larvae stained by WISH against the intestinal marker fabp2a. The expression of fabp2a (blue arrow) is decreased in nol9^{sa1022/sa1022} (n = 11) compared to wild-type siblings (n = 32). (D) Alcian blue staining, showing normal formation of the jaw cartilage elements Meckel’s (m), palatoquadrate (pq), ceratohyal (ch) and ceratobranchial (cb) in both nol9^{sa1022/sa1022} mutants (n = 23) and wt siblings (n = 54) at 120 hpf. Ventral view with anterior to the left.

Acknowledgments

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