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Fig. 5
Live imaging of cell division and death in the developing epidermis. (A) Still images from a time lapse movie of cell division in the basal Gal4 line (zc1036A) driving expression of fluorescently labeled microtubules Tg(UAS:Gtuba2). (B) Image of a cell being extruded from the epidermis of a live zebrafish in the periderm Gal4 line (zc1044A) driving expression of fluorescently labeled F-actin Tg(UAS:Lifeact-EGFP). (C,D) Expression of Tg(UAS:nCas9n-2A-EGFP-CAAX) in zc1036A (C) and zc0144A (D) GAL4 lines. Scale bars: 20 μm in A, 25 μm in B, 25 μm in C,D.
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Full text @ J. Cell Sci.