ZFIN Image: Rydeen et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Gir mutants and gir+c1 embryos fail to add ventricular cardiomyocytes and have ectopic cardiomyocytes.

(A-H) IHC of hearts from control, Cyp26c1-depleted, gir mutant, and gir+c1 Tg(myl7:DsRed-NLS) embryos at 48 and 72 hpf. (I-P) Hearts from control, Cyp26c1-depleted, gir mutant, and gir+c1 Tg(myl7:EGFP) embryos at 48 and 72 hpf. Arrows denote ectopic cardiomyocytes. Ectopic cardiomyocytes were observed in 0/74 control, 1/80 Cyp26c1-depleted, 8/29 gir, and 7/31 gir+c1 at 48 hpf. (Q) Graph depicting ventricular cardiomyocyte counts at 48 and 72 hpf (n = 10 per group). (R) Graph depicting atrial cardiomyocyte counts at 48 hpf (n = 10 per group). (S) Graph depicting atrial cardiomyocyte counts at 48 hpf (n = 10 per group) in control and Cyp26-deficient embryos. (T) Graph of ventricular addition to the OFT (n = 10 per group). (U-X) Control, cyp26c1 MO injected, gir, and gir+c1 Tg(myl7:Kaede) embryo hearts at 48 hpf after photoconversion at 36 hpf. Brackets indicate ventricular addition (green only). Error bars are SEM, asterisks denote p<0.05 compared to controls by Student’s t-test. All images are frontal views with anterior up; n>20 embryos for (U-X). Scale bars: 50 mm.

Acknowledgments

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