ZFIN Image: Shih et al., 2016, Fig. 8

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Figure Caption

Fig. 8

Contraction-dependent myofibril damage to the slow muscle of ttn.2^xu064. (A) Slow muscle defects in ttn.2^xu064 embryos. Immunostaining for Myosin and Phalloidin staining to label Actin filaments in 2 dpf embryos. Arrows indicate the two rod-like structures enclosing the damaged myofibril region. (B) Fast muscle is intact in ttn.2^xu064. Immunostaining with antibodies against Tropomyosin and Myomesin or staining with Phalloidin in 2 dpf embryos. (C) ttn.2^xu064 mutants and siblings treated with 1% tricaine for 24 h or 0.07% bleach at 1 dpf for 5 min were fixed and immunostained for Myosin at 2 dpf. Scale bars: 5 μm.

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Acknowledgments

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