Fig. S2

Fig. S2

tbx2a/b splice MO efficiency verification using RT-PCR. (A) Whole mount in situ hybridization reveals that tbx2a knockdown by tbx2a splice-blocking MO causes a decrease in the DL segment, marked by slc12a3. The tbx2a splice MO targets the splice acceptor site of intron 1, causing a fusion of exon 1 and exon 3. This change can be resolved by gel electrophoresis of RT-PCR wild-type and tbx2a splice morphant products. Sequencing revealed that the lower band present in morphants (asterisk) is the excised exon 2. (B) Similarly to effects of the tbx2a splice-blocking MO, the tbx2b splice-blocking MO causes a decrease in the DL segment. The tbx2b splice MO was designed to block the exon 3 splice donor site, resulting in an excision of exon 3. Sequencing revealed that the lower band present only in tbx2b splice morphant RT-PCR product is exon 3 from the tbx2b gene.