ZFIN Image: Drummond et al., 2017, Fig. S1

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Figure Caption

Fig. S1

tbx2a/b are expressed early in development and remain in the distal region of the developing pronephros. (A) Whole mount in situ hybridization using riboprobes for tbx2a and tbx2b reveal that these genes are expressed in the precursor tissue of the pronephros, the intermediate mesoderm, as early as the 8 ss. Both tbx2a and tbx2b continue to be expressed in the developing distal region of the pronephros through the 28 ss (24 hpf). (B) Sense probes for tbx2a and tbx2b applied to 28 ss wild-type embryos demonstrate that tbx2a/b expression in the pronephros is specific, rather than an artifact of the staining technique.

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Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.