ZFIN Image: Drummond et al., 2017, Fig. 8

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Fig. 8

tbx2a/b are downstream of retinoic acid in the nephrogenesis pathway. Wild-type embryos were treated with exogenous RA at 3 dosages from 90% epiboly to the 28 ss. Wild-type embryos were also subjected to an RA inhibitor (DEAB) at 1.6×10^-7 M at either 50% epiboly or 90% epiboly until fixation at 24 hpf. Whole mount in situ hybridization was then performed using riboprobes for tbx2a and tbx2b. Both the tbx2a and tbx2b domains decreased with at all three doses of RA, with total abrogation of expression occurring at 1×10^-6 M. In contrast, DEAB treatment induced an expansion in the tbx2a/b domains at both treatment time points. This suggests that RA negatively regulates tbx2a/b expression, either directly or indirectly, in the development of the distal pronephros. At least 20 representative embryos were examined in each treatment.

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Acknowledgments

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Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.