ZFIN Image: Drummond et al., 2017, Fig. 6

Image

Figure Caption

Fig. 6

tbx2a/b are necessary and sufficient to inhibit CS cellular expansion. (A) Dorsal images of wild-type 28 ss embryos injected with tbx2a and tbx2b cRNA show that tbx2a/b overexpression results in a decrease in the number of stc1-expressing CS cells. Rescue studies were conducted by co-injecting wild-type with either tbx2a cRNA and tbx2a MO or tbx2b cRNA and tbx2b MO and fby^c144 mutants with tbx2b cRNA. In all three conditions, the CS was successfully rescued. (B) The number of CS cells in each nephron was counted for at least 10 representative embryos from each condition. The number of CS cells in embryos injected with tbx2a cRNA and tbx2b cRNA were significantly lower than wild-type embryos. In contrast, the number of CS cells in rescue study embryos was not significantly different than wild-type embryos. (*p<0.05, **p<0.001, N.S.=not significant). Abbreviations: cRNA (capped RNA), CS (corpuscle of Stannius).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.