ZFIN Image: Begay et al., 2016, Fig. 4

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Figure Caption

Fig. 4

MO Knockdown of flncb in Zebrafish Showing a Cardiac Phenotype

(A) Black bars: percentage of surviving embryos with severe cardiac phenotype at 72 h post-fertilization (hpf) for uninjected (n = 411), p53 morpholino (MO)-injected (n = 139), 25-N+p53 control MO-injected (n = 351), and filamin Cb (flncb)+p53 MO-injected (n = 241); *p = 1.04 � 10^-83 (for comparison of uninjected heart vs. flncb+p53 MO using chi-square testing). Gray bars: percentage of surviving embryos at 7 days for uninjected (n = 411), p53 MO-injected (n = 139), 25-N+p53 control MO-injected (n = 351), and flncb+p53 MO-injected (n = 164); ?p = 1.65 � 10^-19 (for comparison of uninjected embryo vs. flncb+p53 MO using chi-square testing). (B) Representative zebrafish heart pictures for embryo uninjected and flncb MO coinjected with p53 MO zebrafish embryos at 48 hpf. The flncb+p53 MO-injected zebrafish show cardiac phenotype (pericardial edema [P]), and GFP hearts showing less heart looping, elongated atrium, pericardial edema, and a truncated ventricle. (C) Representative zebrafish heart pictures for embryo uninjected and flncb MO coinjected with p53 MO zebrafish embryos at 72 hpf. Among the flncb+p53 MO-injected zebrafish, they show cardiac phenotype, and GFP hearts further show less heart looping, elongated atrium, pericardial edema, and a truncated ventricle.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JACC Basic Transl Sci